1,2,3 triazole effect antimicrobial
Introduction:

1,2,3 Triazoles  is  important type of heterocyclic compound successful application in medicine and as agrochemicals[1]

 Number of five membered nitrogen containing heterocy-cles, have turned out to be potential chemotherapeutic and pharmacotherapeutic agents. The biological profile of tria-zoles derivatives is very extensive[2]

 symmetrical triazole moiety are reported to show a broad spectrum of pharmacological activities such as antibacte-rial, antifungal, antimicrobial, antimycobacte-rial, antioxidant, analgesic, antipyretic, antican-cer, anticonvulsant and anti-inflammatoryactivi-ties[3]


 Number of five membered nitrogen containing heterocy-cles, have turned out to be potential chemotherapeutic and pharmacotherapeutic agents. The biological profile of tria-zoles derivatives is very extensive. Compounds bearing a symmetrical triazole moiety are reported to show a broad spectrum of pharmacological activities such as antibacte-rial, antifungal, antimicrobial, antimycobacte-rial, antioxidant, analgesic, antipyretic, antican-cer, anticonvulsant23 and anti-inflammatory  activi-ties. These observations prompted us to synthesize N-[5-(substitutedphenyl)-1H-1,2,3-triazol-1-yl]isonicotinamide derivatives and 4-(substituted benzylideneamino)-5- pyridin-4-yl)-4H-1,2,4-triazole-3-thiol derivatives using isonicotinic acid as starting material by two different schemes. In the present paper, we describe the synthesis, charac-terization and antimicrobial as well as anti-inflammatory activities of the synthesized triazole derivatives. The struc-ture assigned to compounds was substantiated by their ana-lytical and other spectral data



Most of the nitrogen-containing molecules are pharmacologically very active which can be attributed to the fact that nitrogenous compounds are part and parcel of the biomolecular diversity [1-7]. Amongst the pharmacologically active nitrogenous compounds, a large number of 1,2,3-triazoles and their derivatives attracted considerable attention for the past few decades due to their chemotherapeutical value. Many 1,2,3-triazoles including bis-triazoles, are found to be potent antimicrobial analgesic, anti-inflammatory, local anesthetic anti-convulsant, anti-neoplastic, anti-malarial, and antiviral agents[4]

Some of them exhibited anti-proliferative, anticancer activity, and several are used as DNA cleaving agents and potassium channel activators. Such type of diverse biological functions is also reported with a variety of bis-triazoles. The ‘click chemistry’ approach has been the most widely used method for the synthesis of libraries of a large number of biologically active molecular

frameworks particularly for the regioselective synthesis of 1,2,3-triazoles, which involves the copper(I)- catalyzed cycloaddition reaction between azides and terminal alkynes (CuAAC). This reaction has been termed as the cream of the cropof click reactionsand has found application in various facets of drug discovery as it enables a modular approach to generate novel

pharmacophores utilizing a collection of reliable chemical reactions [11,12]. Thus, the development of the copper(I)-catalyzed triazole click chemistryhas led to many interesting applications including the synthesis, medicinal chemistry, molecular biology, and material science. The bioorthogonality of azide and alkynes [13] has allowed the use of their [3 + 2] cycloaddition in various

biological applications including target guided synthesis [14] and activity-based protein profiling [15]. Of particular interest would be the dimeric heterocyclebased ligands which are designed for specific target interactions. Various approaches reported for the synthesis of biologically relevant bis-triazoles include Cu(I)- catalyzed 1,3-dipolar cycloaddition of monoazides with

diacetylenes or that of monoacetylenes with diazides. For example, the synthesis of bis-triazoles is reported by the reactions of bis(azidomethyl)benzenes with several substituted acetylenes [16]. Recently, much attention has been paid toward the synthesis and pharmacological evaluation of triazoles and bis-triazoles as potent HIV-1 protease inhibitors [17,18] and size-specific ligands for mRNA Hairpin loops [19], respectively. Keeping into consideration the tremendous biological potence of triazoles

and bis-triazoles in general and the antimicrobial activity in particular, we, in our continuous endeavor toward the synthesis of pharmacologically active molecules, designed the synthesis of novel unsymmetrical bis-1,2,3-triazoles and then evaluated them for antimicrobial activities. The biological results obtained were very interesting and revealed most of the synthesized molecules to be potent antimicrobials


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